OPTIMIZATION OF COLONY POLYMERASE CHAIN REACTION FOR THE 16SRRNA OF DIFFERENT STRAINS OF ESCHERICHIA COLI

Objective: This work aimed to enhance colony polymerase chain reaction (PCR) for the 16S rRNA of several Escherichia coli strains. Methods: The isolation E. is done from gut chicken and soil. Then, we optimized condition PCR amplification 16s ribosomal RNA. We successfully designed primer 3 RNA made dilution solution with grade water that 1:10. Moreover, finally, a 20 μL contains master mix our isolated forward reverse base amplification. After conventional PCR, amplified was then run on Gel obtain desired bands. And finally saw bands in Doc picture. Results: Our result shows technique an efficient quick method than other existing methods are too costly, tedious, time-consuming procedures deter their exploitation various experimentations identification Conclusion: study concluded can be without extraction purification total genomic DNA bacterial using PCR. Therefore, by designing primers species, evaluate types mutations, strain detection, antibiotic resistance.